Glucose is an essential energy source for germ cells. The fatty acids in the spermatozoa have been suggested to be important for the viability, maturity, and function of spermatozoa. The amount of cholesterol in the seminiferous tubules is also known to be inversely correlated with spermatogenic activity. However, these substances are little or not produced in the testis and must be imported from the bloodstream. In this study, we analyzed changes in selected blood biochemical parameters to determine whether there is a link between them and the spermatogenic activity variation in normal mink (Mustela vison) and in mink with spontaneous autoimmune orchitis (AIO) in which spermatogenesis is absent. Our results showed that glucose levels were significantly (p<0.005) lower during the breeding season compared to out of the breeding season in normal mink. In mink with AIO, glucose level was 10 times higher (p<0.001) compared to normal mink in March. TC and TG showed no significant changes. HDL-C levels were lower and LDL-C levels higher during the breeding season compared to out of it in normal mink. HDL-C and LDL-C showed no significant changes in AIO compared to normal mink. The results showed here suggest that the blood levels of certain biochemical parameters are influenced by spermatogenesis in mink.