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Wiley, FEBS Letters, 1-2(578), p. 80-84

DOI: 10.1016/j.febslet.2004.10.074

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Glutamate-evoked redox state alterations are involved in tissue transglutaminase upregulation in primary astrocyte cultures

Journal article published in 2004 by A. Campisi, D. Caccamo, G. Li Volti, M. Currò ORCID, G. Parisi, R. Avola, A. Vanella, R. Ientile
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The aim of this study was to evaluate the involvement of oxidative stress in glutamate-evoked transglutaminase (TGase) upregulation in astrocyte cultures (14 DIV). A 24 h exposure to glutamate caused a dose-dependent depletion of glutathione intracellular content and increased the ROS production in cell cultures. These effects were receptor-mediated, as demonstrated by inhibition with GYKI 52466. The pre-incubation with glutathione ethyl ester or cysteamine recovered oxidative status and was effective in significantly reducing glutamate-increased tissue TGase. These data suggest that tissue TGase upregulation may be part of a biochemical response to oxidative stress induced by a prolonged exposure of astrocyte cultures to glutamate.