Bambusicola, gen. nov., is introduced for four new saprobic taxa from culms of bamboos in northern Thailand. One species, chosen as the generic type, has both sexual and asexual morphs, one species has the sexual morph only and two species have the asexual morph only. The four new species, Bambusicola massarinia, B. bambusae, B. irregulispora and B. splendida are illustrated and described. Isolates of each species were sequenced using LSU, SSU and ITS genes and phylogenetic analysis shows the genus to be a distinct clade in the suborder “Massarineae”. Morphological differences between Bambusicola and some related genera are noted. Bambusicola is characterized by small, cone-shaped ascomata, slightly broad and fusiform ascospores and a coelomycete asexual morph with light brown conidia. Bambusicola clusters in Trematosphaeriaceae, but the closeness of this relationship cannot be resolved.