Published in

American Chemical Society, Journal of Proteome Research, 4(4), p. 1339-1346, 2005

DOI: 10.1021/pr050048h

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Global Phosphoproteome of HT-29 Human Colon Adenocarcinoma Cells

Journal article published in 2005 by Ji-Eun Kim, Steven R. Tannenbaum, Forest M. White ORCID
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

We identified 238 phosphorylation sites on 116 proteins from human colon adenocarcinoma cells by immobilized metal affinity chromatography combined with liquid chromatography-tandem mass spectrometry analysis. Phosphorylation sites identified in this work were compared to known kinase motifs using bioinformatics tools to estimate kinase activity and functionality. Phosphorylation sites identified in this work may be utilized to assess the activity of selected kinases from different cell states and exogenous stimuli.