<i>Aims:</i> Previously we reported an association between levels of anti-Hsp60 autoantibodies and interleukin-6 (IL-6) –174 SNP in Finnish population. The aim of this study was to investigate the same association in an independent population and to study four recently described SNP in <i>IL-6</i>. <i>Materials and Methods:</i> 313 healthy Hungarian subjects were recruited and genotyped for IL-6 –174(G→C), –9316(T→C), –1363(G→T), +1753(C→G), +2954(G→C). IgG antibodies to Hsp60 were measured by ELISA. LD between SNPs was computed by Haploview 3.2 software. <i>Results:</i> A strong association between IL-6 –174 polymorphism and anti-Hsp60 autoantibody levels was observed. Carriers of –174 CC genotype had significantly lower levels of anti-Hsp60 (p = 0.0052). Eight haplotypes were observed with five SNP-s and autoantibody levels in individuals carrying the most common haplotype (containing allele C of –174) were significantly lower than in all other genotype combinations (p = 0.026). <i>Conclusions:</i> Allele C of –174 promoter polymorphism of the IL-6 gene was repeatedly shown to be associated with low anti-Hsp60 autoantibody levels. Strong linkage in the IL-6 gene was observed and the most frequent haplotype containing the –174 C allele was significantly associated with autoantibody levels. Since the –174 SNP of IL-6 is a functional polymorphism, our results indicate for a direct regulatory effect of IL-6 genotypes in the determination of autoantibody levels.