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Wiley, Macromolecular Rapid Communications, 15(29), p. 1293-1297, 2008

DOI: 10.1002/marc.200800248

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Synthesis of Branched Polyglucans by the Tandem Action of Potato Phosphorylase andDeinococcus geothermalisGlycogen Branching Enzyme

Journal article published in 2008 by Jeroen van der Vlist, Marta Palomo Reixach, Marc van der Maarel, Lubbert Dijkhuizen, Arend Jan Schouten, Katja Loos ORCID
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This paper is available in a repository.

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Abstract

An enzymatic tandem reaction is described in which the enzymes phosphorylase and Deinococcus geothermalis glycogen branching enzyme (Dg GBE) catalyze the synthesis of branched polyglucans from glucose-1-phosphate (G-1-P). Phosphorylase consumes G-1-P and polymerizes linear amylose while Dg GBE introduces branching points on the α-(1→6) positions by reshuffling short oligosaccharides. The resulting branched polyglucans have an unusually high degree of branching of 11%.