We present an agent-based distributed decision support system for the diagnosis and prognosis of brain tumors developed by the HEALTHAGENTS project. HEALTHAGENTS is a European Union funded research project, which aims to enhance the classification of brain tumours using such a decision support system based on intelligent agents to securely connect a network of clinical centres. The HEALTHAGENTS system is implementing novel pattern recognition discrimination methods, in order to analyse in vivo Magnetic Resonance Spectroscopy (MRS) and ex vivo/in vitro High Resolution Magic Angle Spinning Nuclear Magnetic Resonance (HR-MAS) and DNA micro-array data. HEALTHAGENTS intends not only to apply forefront agent technology to the biomedical field, but also develop the HEALTHAGENTS network, a globally distributed information and knowledge repository for brain tumour diagnosis and prognosis.