An organic field-effect transistor (OFET) integrating bacteriorhodopsin (bR) nano-assembled lamellae is proposed for an in depth study of the proton translocation processes occurring as the bio-electronic device is exposed either to light or to low concentrations of general anesthetic vapors. The study involves the morphological, structural, electrical and spectroscopic characterizations, necessary to assess the functional properties of the device as well as the bR biological activity once integrated into the functional bio-interlayer (FBI)-OFET structure. The electronic transduction of the protons photo-translocation is shown as a current increase in the p-type channel, only when the device is irradiated with photons known to trigger the bR photocycle, while Raman spectroscopy reveals an associated C=C isomer-switch. Notably, higher energy photons bring the cis isomer back to its trans-form, switching the proton pumping process off. The investigation is extended also to the study of a PM FBI-OFET exposed to volatile general anesthetics such as halothane. In this case an electronic current increase is seen upon exposure to low, clinically relevant, concentration of anesthetics while no evidence of isomer-switch is observed. The study of the direct electronic detection of the two different externally triggered proton translocation effects allows gathering insights into the underpinning different bR molecular switching processes.