Wood toughness is a mechanical property of interest in structural design where the load impact must be considered, finding a strong application in bridges, however, not an integral part of the mechanical properties commonly investigated in the characterization of this material. This study aimed to investigate with the aid of variance analysis, the influence of growth ring orientation to obtain toughness for Angelim Saia (Parkia pendula), Eucalyptus grandis, Pinus elliottii and Cor-ymbia citriodora wood species, considering three different positions, so that the orientation causes tensile strength in fibers near the pitch, near the bark, and in the radial direction. The results of the statistical analysis revealed that there was no significance in the orientation of the growth rings to obtain the toughness of the wood species investigated, where the highest values were for Corymbia citriodora and the least values were for Pinus elliottii.