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Sociedade Brasileira de Química, Química Nova, 1(36), p. 52-58

DOI: 10.1590/s0100-40422013000100010

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Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel process

Journal article published in 2013 by Carvalho,Nayara Bezerra, Fricks,Alini T., Nayara Bezerra Carvalho, José Murillo P. Barbosa, Barbosa,José Murillo P., Maria Vanessa S. Oliveira, Oliveira,Maria Vanessa S., Alini T. Fricks ORCID, Álvaro S. Lima, Lima,Álvaro S., Soares,Cleide Mara F., Cleide Mara F. Soares
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

This work presents biochemical characterization of a lipase from a new strain of Bacillus sp. ITP-001, immobilized using a sol gel process (IB). The results from the biochemical characterization of IB showed increased activity for hydrolysis, with 526.63 U g-1 at pH 5.0 and 80 ºC, and thermal stability at 37 ºC. Enzymatic activity was stimulated by ions such as EDTA, Fe+3, Mn+2, Zn+2, and Ca+2, and in various organic solvents. Kinetic parameters obtained for the IB were Km = 14.62 mM, and Vmax = 0.102 mM min-1 g-1. The results of biochemical characterization revealed the improved catalytic properties of IB.