The data presented describe the development of an enzymatic process in vegetable oils. Six bacterial lipases were tested for their ability to hydrolyze. For each lipase assay, the p-NPP method was applied to obtain maximum enzymatic activities. The lipase from Burkholderia cepacia (lipase B-10) was the most effective in buriti oil, releasing 4840 μmol p-NP mL -1. The lipase from Klebsiella variicola (lipase B-22) was superior in passion fruit oil, releasing 4140 μmol p-NP mL -1 and also in babassu palm oil, releasing 2934 μmol p-NP mL -1. Research into the bioprocessing of oils aims to provide added value for this regional raw material.