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Optica, Optics Express, 22(17), p. 19763, 2009

DOI: 10.1364/oe.17.019763

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Three distinct sarcomeric patterns of skeletal muscle revealed by SHG and TPEF microscopy.

Journal article published in 2009 by Gaëlle Recher ORCID, Denis Rouède, Patrick Richard ORCID, Antoine Simon, Jean-Jacques Bellanger, François Tiaho
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We have extensively characterized the sarcomeric SHG signal as a function of animal species (rat versus xenopus), age (adult versus larval) and tissue preparation (fixed or fresh) and we found that the main feature of this signal is a single peak per mature sarcomere (about 85% of all sarcomeres). The remaining (15%) was found to be either double peak per mature sarcomere or mini sarcomeres (half of a sarcomere) using alpha-actinin immuno detection of the Z-band. The mini sarcomeres are often found in region of pitchfork-like SHG pattern. We suggest that double peak SHG pattern could indicate regions of sarcomeric proteolysis whereas pitchfork-like SHG pattern could reveal sarcomeric assembly.