Dissemin is shutting down on January 1st, 2025

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Public Library of Science, PLoS ONE, 9(10), p. e0139080, 2015

DOI: 10.1371/journal.pone.0139080

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Serendipitous Meta-Transcriptomics: The Fungal Community of Norway Spruce (Picea abies)

Journal article published in 2015 by Nicolas Delhomme, Görel Sundström, Neda Zamani, Henrik Lantz ORCID, Yao-Cheng Lin, Torgeir Rhoden Hvidsten, Marc P. Hoppner, Marc P. Höppner, Patric Jern, Yves Van de Peer, Joakim Lundeberg, Manfred G. Grabherr, Nathaniel R. Street
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

After performing de novo transcript assembly of >1 billion RNA-Sequencing reads obtained from 22 samples of different Norway spruce (Picea abies) tissues that were not surface sterilized, we found that assembled sequences captured a mix of plant, lichen, and fungal transcripts. The latter were likely expressed by endophytic and epiphytic symbionts, indicating that these organisms were present, alive, and metabolically active. Here, we show that these serendipitously sequenced transcripts need not be considered merely as contamination, as is common, but that they provide insight into the plant's phyllosphere. Notably, we could classify these transcripts as originating predominantly fromDothideomycetes and Leotiomycetes species, with functional annotation of gene families indicating active growth and metabolism, with particular regards to glucose intake and processing, as well as gene regulation. © 2015 Delhomme et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.