Published in
Nature Research, Nature Methods, 5(12), p. 404-406, 2015
DOI: 10.1038/nmeth.3323
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CIDRE: an illumination-correction method for optical microscopy
Journal article published in 2015 by
Kevin Smith 
,
Yunpeng Li,
Filippo Piccinini ,
Gabor Csucs,
Csaba Balazs,
Alessandro Bevilacqua ,
Péter Horváth,
Csaba Balázsi
,
Yunpeng Li,
Filippo Piccinini ,
Gabor Csucs,
Csaba Balazs,
Alessandro Bevilacqua ,
Péter Horváth,
Csaba Balázsi
This paper is made freely available by the publisher.
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Abstract
Uneven illumination affects every image acquired by a microscope. It is often overlooked, but it can introduce considerable bias to image measurements. The most reliable correction methods require special reference images, and retrospective alternatives do not fully model the correction process. Our approach overcomes these issues for most optical microscopy applications without the need for reference images.