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Elsevier Masson, Analytical Biochemistry, 2(388), p. 348-350

DOI: 10.1016/j.ab.2009.03.001

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Validation of an appropriate reference gene for normalization of reverse transcription–quantitative polymerase chain reaction data from rectal cancer biopsies

Journal article published in 2009 by Alexandre Ho-Pun-Cheung, Caroline Bascoul-Mollevi, Eric Assenat, Frédéric Bibeau, Florence Boissière-Michot ORCID, Dominic Cellier, Marc Ychou, Evelyne Lopez-Crapez
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Gene expression quantification using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) requires data normalization using an invariable reference gene. Here we assessed the stability of 15 housekeeping genes in 31 tumor and normal rectal samples to validate a reliable reference gene for rectal cancer studies. Our data show that 18S and 28S RNA are stably expressed in all samples. Moreover, when used for normalization, 18S, but not 28S, greatly reduced unspecific variations of gene expression due to RNA degradation. These results demonstrate that 18S is an appropriate reference gene for normalization of RT-qPCR data from rectal cancer samples.