Wiley, Yeast, 15(14), p. 1373-1386, 1998
DOI: 10.1002/(sici)1097-0061(199811)14:15<1373::aid-yea332>3.0.co;2-1
Wiley, Yeast, 15(14), p. 1373-1386
DOI: 10.1002/(sici)1097-0061(199811)14:15<1373::aid-yea332>3.3.co;2-t
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The ACO3 gene, which encodes one of the acyl-CoA oxidase isoenzymes, was isolated from the alkane-utilizing yeast Yarrowia lipolytica as a 10 kb genomic fragment. It was sequenced and found to encode a 701-amino acid protein very similar to other ACOs, 67·5% identical to Y. lipolytica Aco1p and about 40% identical to S. cerevisiae Pox1p. Haploid strains with a disrupted allele were able to grow on fatty acids. The levels of acyl-CoA oxidase activity in the ACO3 deleted strain, in an ACO1 deleted strain and in the wild-type strain, suggested that ACO3 encodes a short chain acyl-CoA oxidase isoenzyme. This narrow substrate spectrum was confirmed by expression of Aco3p in E. coli. © 1998 John Wiley & Sons, Ltd.