The Externally Bonded Reinforcement (EBR) technique using Carbon Fiber-Reinforced Polymers (CFRP) has been commonly used to strengthen concrete structures in flexure. The use of prestressed CFRP material offers several advantages well-reported in the literature. Regardless of such as benefits, several studies on different topics are missing. The present work intends to contribute to the knowledge of two commercially available systems that differ on the type of anchorage: (i) the Mechanical Anchorage (MA), and (ii) the Gradient Anchorage (GA). For that purpose, an experimental program was carried out with twelve slabs monotonically tested under displacement control up to failure by using a four-point bending test configuration. The effect of type of anchorage system (MA and GA), prestrain level (0 and 0.4%), width (50 mm and 80 mm) and thickness (1.2 mm and 1.4 mm) of the CFRP laminate, and the surface preparation (grinded and sandblasted) on the flexural response were the main studied parameters. Better performance was observed for the slabs: (i) with prestressed laminates, (ii) for the MA system, and (iii) with sandblasted surface preparation.