Published in
BioMed Central, Veterinary Research, 1(46), 2015
DOI: 10.1186/s13567-015-0250-8
Links
- ORCID
- BioMed Central
- [www.veterinaryresearch.org] | PDF
- [espace.library.uq.edu.au]
- [dx.doi.org] | PDF
- [hal.archives-ouvertes.fr] | PDF
- [www.research.ed.ac.uk] | PDF
- [www.pure.ed.ac.uk] | PDF
- [europepmc.org] | PDF
- [www.researchgate.net] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
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Assessment of population genetic structure in the arbovirus vector midge, Culicoides brevitarsis (Diptera: Ceratopogonidae), using multi-locus DNA microsatellites
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Abstract
Abstract Bluetongue virus (BTV) is a major pathogen of ruminants that is transmitted by biting midges ( Culicoides spp.). Australian BTV serotypes have origins in Asia and are distributed across the continent into two distinct episystems, one in the north and another in the east. Culicoides brevitarsis is the major vector of BTV in Australia and is distributed across the entire geographic range of the virus. Here, we describe the isolation and use of DNA microsatellites and gauge their ability to determine population genetic connectivity of C. brevitarsis within Australia and with countries to the north. Eleven DNA microsatellite markers were isolated using a novel genomic enrichment method and identified as useful for genetic analyses of sampled populations in Australia, northern Papua New Guinea (PNG) and Timor-Leste. Significant ( P