Published in
Humana Press, Methods in Molecular Biology, p. 269-275, 2008
DOI: 10.1007/978-1-60327-058-8_17
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A medium or high throughput protein refolding assay
Journal article published in 2008 by
Nathan P. Cowieson,
Beth Wensley,
Gautier Robin,
Gregor Guncar,
Jade K. Forwood 
,
David A. Hume,
Bostjan Kobe,
Jennifer L. Martin
,
David A. Hume,
Bostjan Kobe,
Jennifer L. Martin
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Abstract
Expression of insoluble protein in E. Coli is a major bottleneck of high throughput structural biology projects. Refolding proteins into native conformations from inclusion bodies could significantly increase the number of protein targets that can be taken on to structural studies.