An investigation of intracellular localization of Co-phthalocyanines is reported. The Raman images of K562 cells stained with phthalocyanine were acquired. To understand the peculiarities of the Raman images, measurements were performed at different z-axis positions. The intracellular concentration of phthalocyanine was estimated. A colocalization study was carried out using the fluorescence probes FITC-dextran and acridine orange by means of Raman and fluorescence microscopy. Partial colocalization with both probes was revealed.