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International Union of Crystallography, Acta Crystallographica Section D: Biological Crystallography, 8(57), p. 1138-1140, 2001

DOI: 10.1107/s090744490100748x

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Cloning, purification and characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus subtilis

Journal article published in 2001 by Edward J. Taylor, Simon J. Charnock, John Colby, Gideon J. Davies ORCID, Gary W. Black
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The enzyme 6-phospho-3-hexulose isomerase (YckF) from Bacillus subtilis has been prepared and crystallized in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop method at 291 K using polyethylene glycol 2000 monomethylether as precipitant. They diffract beyond 1.7 A using an in-house Cu Kalpha source and belong to either space group P6(5)22 or P6(1)22, with unit-cell parameters a = b = 72.4, c = 241.2 A, and have two molecules of YckF in the asymmetric unit.