Wiley, ELECTROPHORESIS, 2(22), p. 249-257, 2001
DOI: 10.1002/1522-2683(200101)22:2<249::aid-elps249>3.0.co;2-4
Wiley-VCH Verlag, ELECTROPHORESIS, 2(22), p. 249-257
DOI: 10.1002/1522-2683(200101)22:2<249::aid-elps249>3.3.co;2-w
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The filter-chamber array presented here enables a real-time parallel analysis of three different samples on beads in a volume of 3 nL, on a 1 cm2 chip. The filter-chamber array is a system containing three filter-chambers, three passive valves at the inlet channels and a common outlet. The design enables parallel sample handling and time-controlled analysis. The device is microfabricated in silicon and sealed with a Pyrex lid to enable real-time analysis. Single nucleotide polymorphism analysis by using pyrosequencing has successfully been performed in single filter-chamber devices. The passive valves consist of plasma-deposited octafluorocyclobutane and show a much higher resistance towards water and surface-active solutions than previous hydrophobic patches. The device is not sensitive to gas bubbles, clogging is rare and reversible, and the filter-chamber array is reusable. More complex (bio)chemical reactions on beads can be performed in the devices with passive valves than in the devices without valves.