Published in

Humana Press, Methods in Molecular Biology, p. 259-266

DOI: 10.1007/978-1-59745-538-1_16



Export citation

Search in Google Scholar

Comparative genomic hybridization: DNA preparation for microarray fabrication.

Journal article published in 2009 by Richard Redon ORCID, Diane Rigler, Nigel P. Carter
This paper is available in a repository.
This paper is available in a repository.

Full text: Download

Green circle
Preprint: archiving allowed
Green circle
Postprint: archiving allowed
Red circle
Published version: archiving forbidden
Data provided by SHERPA/RoMEO


The spatial resolution of microarray-based comparative genomic hybridization (array-CGH) is dependent on the length and density of target DNA sequences covering the chromosomal region of interest. Here we describe the methods developed at the Wellcome Trust Sanger Institute (Cambridge, UK) to construct microarrays composed of large-insert clones available through genome sequencing projects. These methods are applicable to Bacterial and Phage Artificial Chromosomes (BAC and PAC) as well as fosmid and cosmid clones. The protocols are scalable for the construction of microarrays composed of several hundreds up to several ten thousands clones.