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Elsevier, Veterinary Immunology and Immunopathology, 3-4(121), p. 199-205

DOI: 10.1016/j.vetimm.2007.09.010

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Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets

Journal article published in 2008 by Jason Brunt ORCID, Rasmus Hansen, Derek J. Jamieson, Brian Austin
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

The response of rainbowtrout (Oncorhynchusmykiss, Walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (APR). Proteomicanalysis by two-dimensional electrophoresis (2D) concurrently with mass spectrometry was used to detect APR related proteins in rainbowtroutserum following feeding with probioticsAeromonas sobria GC2 and Bacillus sp. JB-1. Three candidate proteins increased following use of GC2, and were putatively identified as NADH dehydrogenase, dystrophin and mKIAA0350. Conversely, one of the proteins, which were induced following use of JB-1 was identified as transferrin.