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Wiley, Angewandte Chemie, 3(113), p. 627-630, 2001

DOI: 10.1002/1521-3757(20010202)113:3<627::aid-ange627>3.0.co;2-f

Wiley, Angewandte Chemie International Edition, 3(40), p. 607-610

DOI: 10.1002/1521-3773(20010202)40:3<607::aid-anie607>3.3.co;2-#

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Insights into the branched-chain formation of mycarose: Methylation catalyzed by an (S)-adenosylmethionine-dependent methyltransferase

Journal article published in 2001 by Huawei Chen, Zongbao Zhao ORCID, Tina M. Hallis, Zhihong Guo, Hung-Wen Liu
This paper is available in a repository.
This paper is available in a repository.

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Abstract

A C-methyltransferase involved in methyl-branch formation in sugars has been characterized for the first time. TylC3, an (S)-adenosylmethylthionine(AdoMet)-dependent enzyme, catalyzes the attachment of a methyl branch [Eq. (1)] in the biosynthesis of L-mycarose, an unusual sugar found in tylosin and as its O-3-methyl derivative in erythromycin. The C-3 methylation proceeds with inversion of configuration and does not require the assistance of any cofactors. The turnover rate is 1.4±0.1 min−1. TDP=thymidine-5′-dihydrogenphosphate.