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Wiley, Angewandte Chemie International Edition, 7(49), p. 1252-1257, 2010

DOI: 10.1002/anie.200906190

Wiley, Angewandte Chemie, 7(122), p. 1274-1279, 2010

DOI: 10.1002/ange.200906190

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Oriented immobilization of farnesylated proteins by the thiol-ene reaction

Journal article published in 2010 by Dirk Weinrich, Po-Chiao Lin, Pascal Jonkheijm, Uyen Tt T. Nguyen, Hendrik Schroder, Christof M. Niemeyer, Kirill Alexandrov ORCID, Roger Goody ORCID, Herbert Waldmann ORCID
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

Anchoring the protein: Proteins were immobilized rapidly under mild conditions by thiol-ene photocoupling between S-farnesyl groups attached to a genetically encodable CAAX-box tetrapeptide sequence (A is aliphatic) at the C terminus of the protein and surface-exposed thiols (see scheme). This method enables the oriented covalent immobilization of proteins directly from expression lysates without additional purification or derivatization steps. © 2010 Wiley-VCH Verlag GmbH & Co. KCaA