Biodiesel expansion is currently limited due to high raw material costs for its produc tion. The potential of using sludge from municipal wastewater treatment plants as an alternative lipid feedstock for biodiesel production was investigated. Experiments were conducted to evaluate the suit ability of four different types of wastewater sludges for biodiesel production. Lipids were extracted from primary, secondary, blended and stabilized sludge in a Soxhlet extractor, using hexane as a solvent. Finally, the lipids were converted by acid catalysis transesterification into their corresponding fatty acid methyl esters (FAMEs) - biodiesel . Results indicated that among four sludge tested, primary sludge achieved the greatest lipids and biodiesel yields. Th e amount of extracted lipids for primary sludge was 25.3% compared to 21.9%, 10.1% and 9.1% (dry wt) for blended, stabilized and secondary sludge, respectively. The FAMEs yields obtained in this study were 13.9%, 10.9%, 2.9% and 1% (dry wt) for primary, blended, secondary and stabilized sludge, respectively. The estimation of annual biodiesel production based on the sludge generated in WWTP of Reus was evaluated, showing that primary sludge consists of 87% of the total biodiesel among the wastewater sludges. Gas chromatography analysis of th e FAMEs revealed a similar fatty acids composition for all sludge tested with a predominance of palmitic acid, stearic acid, oleic acid and linoleic acid. Comparison of sludge fatty acid profile with common biodiesel feedstocks showed their suitability for the production of biodiesel. ; Peer Reviewed ; Postprint (published version)