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American Chemical Society, Chemical Research in Toxicology, 1(28), p. 87-91, 2015

DOI: 10.1021/tx500366a

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Possible Chemical Source of Discrepancy between in Vitro and in Vivo Tests in Nanotoxicology Caused by Strong Adsorption of Buffer Components

Journal article published in 2015 by Arianna Marucco, Federico Catalano, Ivana Fenoglio, Francesco Turci ORCID, Gianmario Martra, Bice Fubini
This paper was not found in any repository; the policy of its publisher is unknown or unclear.
This paper was not found in any repository; the policy of its publisher is unknown or unclear.

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Abstract

In the course of studies of the interaction of proteins with TiO2 nanoparticles we have investigated the role of the medium employed in cellular tests, by measuring the variation of  potential vs. pH in the range 2-9 and bovine serum albumin adsorption on TiO2 P25 in the presence of either HEPES or PBS as buffers, both mimicking the physiological pH, but with unlike chemical nature. The two buffers yield remarkably dissimilar surface charges and protein uptake, i.e. they impart different surface characteristics to the particles which could affect the contact with cells or tissues. This may account for dissimilar toxicological outcomes among in vitro tests and particularly between in vitro vs. in vivo tests, considering the high amount of phosphate ions present in body fluids.