In the present study, anatomical features were used to identify tree species chosen to craft farming tools from the 19th and first half of the 20th century preserved in specimen inventories of the open-air museum in Strážnice, southeastern Moravia (Czech Republic). In total, 701 samples from 337 historical farming tools were obtained from museum specimens. The samples were identified at micro- or macroscopic levels, or both. Results indicated local people used floodplain forest wood to construct farming tools in the study region. Nineteen wood species were identified; from this total, those with higher densities and better mechanical properties were used to manufacture tools, and included predominantly beech, oak, and ash. Softwood species, with lower densities were mainly used for chiseled out implements. We hypothesized regional forest species composition played an essential role in woody species choice, however the species also possessed appropriate properties. Cultivated tree species, such as fruit trees, were employed to create common objects; however, currently, these species are typically applied for special purposes. We concluded an increased number of species were employed in the past for utilitarian purposes, including tree species grown outside local forest boundaries, including cultivated fruit tree species; and species were utilized with good, although perhaps tacit, knowledge of their properties.