Cavitation and agitation generated by lasers in fluid-filled root canals create fluid movement and shear stresses along the root canals walls, enhancing removal of the smear layer and biofilm. When used with sodium hypochlorite and EDTA, laser activation of aqueous fluids can increase the efficiency of debridement and disinfection of root canals. However, the use of forward-firing laser fibers with such solutions poses a risk of driving fluid past the root apex, which could cause postoperative complications. The purpose of this study was to evaluate the mechanism of fluid agitation caused by a novel honeycomb tip. Glass capillary tubes filled with distilled water were used to replicate single-tooth root canals. A 980 nm pulsed diode laser was used with 200 μm diameter plain tips, tube-etched conical tips, and honeycomb tips. To record fluid movements, the tubes were backlit and imaged using a digital camera attached to a microscope. The honeycomb tips generated agitation with fluid movement directed onto the walls, while both the conventional plain fibers and the conical tips created fluid movement largely in a forward direction. The use of honeycomb tips alters the pattern of fluid agitation, and this laterally directed effect might lower the risk of fluid extrusion beyond the apex.