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Instituto Oswaldo Cruz, Ministério da Saúde, Memórias do Instituto Oswaldo Cruz., 2(99), p. 195-197, 2004

DOI: 10.1590/s0074-02762004000200014

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Comparison between splenic and lymph node aspirations as sampling methods for the parasitological detection of Leishmania chagasi infection in dogs

Journal article published in 2004 by Stella Maria Barrouin-Melo ORCID, Lain Pontes de-Carvalho, Barrouin Melo Sm, Daniela Farias Larangeira, Larangeira Df, Joelma Trigo, Washington Luís Conrado dos-Santos ORCID, Paulo Henrique Palis Aguiar, Aguiar Ph, Paulo Henrique Palis Aguiar, dos Santos Wl, Washington Luís Conrado dos-Santos, Lain Pontes-De-Carvalho
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The sensitivities of spleen and lymph node cultures for the diagnosis of canine visceral leishmaniasis were compared in 64 anti-Leishmania antibody positive dogs from an endemic area in Brazil. The sensitivity of spleen cultures for Leishmania detection was 97.9%; in lymph node cultures it was 25%. Positive spleen culture was more frequent (p = 0.048, Fisher's exact probability test) in symptomatic (28 out of 33 animals) than in asymptomatic animals (19 out of 31 animals). These results support the use of spleen instead of lymph node aspiration as the choice method for the parasitological diagnosis of the infection.