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Cold Spring Harbor Laboratory Press, RNA, 4(16), p. 817-827, 2010

DOI: 10.1261/rna.1712910

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LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication

Journal article published in 2010 by Rui Pedro Galão ORCID, Ashwin Chari, Isabel Alves-Rodrigues, Daniela Lobão, Antonio Mas ORCID, Christian Kambach, Utz Fischer, Juana Díez
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

LSm1-7 complexes promote cellular mRNA degradation, in addition to translation and replication of positive-strand RNA viruses such as the Brome mosaic virus (BMV). Yet, how LSm1-7 complexes act on their targets remains elusive. Here, we report that reconstituted recombinant LSm1-7 complexes directly bind to two distinct RNA-target sequences in the BMV genome, a tRNA-like structure at the 3′-untranslated region and two internal A-rich single-stranded regions. Importantly, in vivo analysis shows that these sequences regulate the translation and replication of the BMV genome. Furthermore, both RNA-target sequences resemble those found for Hfq, the LSm counterpart in bacteria, suggesting conservation through evolution. Our results provide the first evidence that LSm1-7 complexes interact directly with viral RNA genomes and open new perspectives in the understanding of LSm1-7 functions.