Dissemin is shutting down on January 1st, 2025

Published in

Sociedade Brasileira de Química, SBQ, Journal of the Brazilian Chemical Society, 6(20), p. 1031-1035, 2009

DOI: 10.1590/s0103-50532009000600005

Links

Tools

Export citation

Search in Google Scholar

Antioxidant capacity and biological activity of essential oil and methanol extract of Conobea scoparioides (Cham. & Schltdl.) Benth

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Question mark in circle
Preprint: policy unknown
Question mark in circle
Postprint: policy unknown
Question mark in circle
Published version: policy unknown
Data provided by SHERPA/RoMEO

Abstract

Os ?leos essenciais da planta sub-aqu?tica Conobea scoparioides (fresca e previamente seca) apresentaram rendimentos de 3,4 e 3,3%, respectivamente. Os principais constituintes identificados foram o ?ter met?lico do timol (39,6 e 47,7%), timol (40,0 e 26,4%), ?-felandreno (12,1 e 14,3%) e p-cimeno (1,5 e 1,7%), totalizando mais de 90% nos referidos ?leos. A concentra??o de seq?estro do radical DPPH (CE50) dos ?leos e extrato foi de 46,7 ? 3,6 ?g mL -1 para a planta fresca (CsO-f), de 56,1 ? 2,4 ?g mL -1 para a planta seca (CsO-d), e de 23,0 ? 2,2 ?g mL -1 para o extrato metan?lico (CsE-d). O valor do extrato ? compar?vel ao BHT (19,8 ? 0,5 ?g mL -1 ), usado como padr?o antioxidante. O valor m?dio dos ?leos ? duas vezes menor, mas igualmente importante como agente antioxidante. O teor de Fen?licos Totais (TP, 124,6 ? 8,7 mg GAE per g) e o Trolox Equivalente (TEAC, 144,1 ? 4,9 mg TE per g) do extrato metan?lico confirmaram a significativa atividade antioxidante de C. scoparioides. Da mesma forma, nos bioensaios com larva de camar?o (Artemia salina) o valor m?dio da concentra??o letal dos ?leos (CL 50 , 7,7 ? 0,3 ?g mL -1 ) foi dez vezes maior que no extrato metan?lico (CL 50 , 77,6 ? 7,1 ?g mL -1 ) mostrando importante atividade biol?gica. ; ABSTRACT: The essential oils of the sub-aquatic plant Conobea scoparioides (fresh and dried previously) showed yields of 3.4 and 3.3%, respectively. The main identified constituents were thymol methyl ether (39.6 and 47.7%), thymol (40.0 and 26.4%), ?-phellandrene (12.1 and 14.3%) and p-cymene (1.5 and 1.7%), totalizing more than 90% of the oils. The DPPH radical scavenging activity (EC50) of the oils and extract was 46.7 ? 3.6 ?g mL -1 in the fresh plant (CsO-f), 56.1 ? 2.4 ?g mL -1 in the dried plant (CsO-d), and 23.0 ? 2.2 ?g mL -1 in the methanol extract. The extract (CsE-d)value is comparable to BHT (19.8 ? 0.5 ?g mL -1 ) used as antioxidant standard. The mean value of the oils is twice smaller but equally important as antioxidant agent. The mean amount of Total Phenolics (TP, 124.6 ? 8.7 mg GAE per g) and the Trolox Equivalent (TEAC, 144.1 ? 4.9 mg TE per g) of the methanol extract confirmed the significant antioxidant activity of C. scoparioides. Similarly, in the brine shrimp bioassay (Artemia salina) the mean value of the lethal concentration for the oils (CL 50 , 7.7 ? 0.3 ?g mL -1 ) was ten times higher than the methanol extract (CL 50 , 77.6 ? 7.1 ?g mL -1 ) showing important biological activity.