Dissemin is shutting down on January 1st, 2025

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Oxford University Press, Nucleic Acids Research, 22(36), p. 7059-7067, 2008

DOI: 10.1093/nar/gkn836

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Reinitiated viral RNA-dependent RNA polymerase resumes replication at a reduced rate

Journal article published in 2008 by Igor D. Vilfan, Andrea Candelli, Susanne Hage, Antti P. Aalto, Minna M. Poranen ORCID, Dennis H. Bamford, Nynke H. Dekker
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

RNA-dependent RNA polymerases (RdRP) form an important class of enzymes that is responsible for genome replication and transcription in RNA viruses and involved in the regulation of RNA interference in plants and fungi. The RdRP kinetics have been extensively studied, but pausing, an important regulatory mechanism for RNA polymerases that has also been implicated in RNA recombination, has not been considered. Here, we report that RdRP experience a dramatic, long-lived decrease in its elongation rate when it is reinitiated following stalling. The rate decrease has an intriguingly weak temperature dependence, is independent of both the nucleotide concentration during stalling and the length of the RNA transcribed prior to stalling; however it is sensitive to RNA structure. This allows us to delineate the potential factors underlying this irreversible conversion of the elongation complex to a less active mode.