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Oxford University Press, The Journal of Clinical Endocrinology & Metabolism, 6(75), p. 1540-1544, 1992

DOI: 10.1210/jcem.75.6.1361194

Oxford University Press (OUP), The Journal of Clinical Endocrinology & Metabolism, 6(75), p. 1540-1544

DOI: 10.1210/jc.75.6.1540

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Binding assay for thyrotropin receptor autoantibodies using the recombinant receptor protein.

Journal article published in 1992 by Stéphane Swillens, P. Niccoli, Jacques E. Dumont, Gilbert Vassart, Marian Ludgate ORCID, Sabine Costagliola
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

We have characterized a transfected Chinese hamster ovary cell line, JP09, which expresses high levels of the human TSH receptor (TSH-R). Based on a theoretical biological activity for TSH of 40 IU/mg, JP09 has approximately 90,000 receptors per cell, having a dissociation constant of 1.64 x 10(3) mU/L or 1.47 x 10(-9) mol/L. We have used JP09 to prepare solubilized TSH-Rs which have formed the basis of a binding assay for thyroid-binding inhibiting immunoglobulins in unfractionated sera. We have compared the JP09 assay with the TRAK assay (which is based on solubilized porcine TSH-R) and found a highly positive correlation between the two assays, r = 0.83 P