Dissemin is shutting down on January 1st, 2025

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Public Library of Science, PLoS ONE, 5(9), p. e97657, 2014

DOI: 10.1371/journal.pone.0097657

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Human Hsp60 with Its Mitochondrial Import Signal Occurs in Solution as Heptamers and Tetradecamers Remarkably Stable over a Wide Range of Concentrations

Journal article published in 2014 by Silvia Vilasi, Everly Conway de Macario, Rita Carrotta, Maria Rosalia Mangione, Claudia Campanella, Fabio Librizzi, Loredana Randazzo, Vincenzo Martorana, Antonella Marino Gammazza, Antonella Marino Gammazza, Maria Grazia Ortore ORCID, Annalisa Vilasi, Gabriella Pocsfalvi ORCID, Giosalba Burgio, Davide Corona and other authors.
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

It has been established that Hsp60 can accumulate in the cytosol in various pathological conditions, including cancer and chronic inflammatory diseases. Part or all of the cytosolic Hsp60 could be naïve, namely, bear the mitochondrial import signal (MIS), but neither the structure nor the in solution oligomeric organization of this cytosolic molecule has still been elucidated. Here we present a detailed study of the structure and self-organization of naïve cytosolic Hsp60 in solution. Results were obtained by different biophysical methods (light and X ray scattering, single molecule spectroscopy and hydrodynamics) that all together allowed us to assay a wide range of concentrations of Hsp60. We found that Naïve Hsp60 in aqueous solution is assembled in very stable heptamers and tetradecamers at all concentrations assayed, without any trace of monomer presence.