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Elsevier, Clinical Biochemistry, 7-8(48), p. 542-545, 2015

DOI: 10.1016/j.clinbiochem.2015.02.006

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Validation of a HPLC-MS/MS assay for the determination of total and unbound concentration of temocillin in human serum.

Journal article published in 2015 by Perrin Ngougni Pokem ORCID, Perrin Ngougni Poken, Ana C. Miranda Bastos, Paul M. Tulkens, Pierre Wallemacq, Françoise Van Bambeke ORCID, Arnaud Capron
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Abstract

OBJECTIVES: The aim of this study was to develop and validate a HPLC-MS/MS assay to determine total and unbound concentrations of temocillin in serum samples. DESIGN AND METHODS: Methanolic protein precipitation and ultrafiltration were used for total and unbound concentration extraction, respectively. Extract was injected into a LC-MS/MS system. Reversed phase chromatography was performed on a phenyl grafted column in gradient mode. Temocillin and internal standard (ticarcillin) were identified in positive electrospray ionization mode using ion transitions of m/z 415.34>339.1 and 385.31>160.3, respectively. RESULTS: Temocillin total and unbound concentration quantification assays were linear over concentrations ranging from 1 to 500mg/L and from 0.5 to 300mg/L, respectively. Both assays presented acceptable intra and inter-assay precision and accuracy