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Elsevier, Journal of Biological Chemistry, 40(286), p. 35299-35307, 2011

DOI: 10.1074/jbc.m111.277137

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Altered Arachidonate Distribution in Macrophages from Caveolin-1 Null Mice Leading to Reduced Eicosanoid Synthesis*

Journal article published in 2011 by Alma M. Astudillo, G. Perez-Chacon, Gema Pérez-Chacón, Clara Meana, David Balgoma, Albert Pol ORCID, Miguel Angel del Pozo ORCID, María A. Balboa ORCID, Jesús Balsinde ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

In this work we have studied the effect of caveolin-1 deficiency on the mechanisms that regulate free arachidonic acid (AA) availability. The results presented here demonstrate that macrophages from caveolin-1-deficient mice exhibit elevated fatty acid incorporation and remodeling and a constitutively increased CoA-independent transacylase activity. Mass spectrometry-based lipidomic analyses reveal stable alterations in the profile of AA distribution among phospholipids, manifested by reduced levels of AA in choline glycerophospholipids but elevated levels in ethanolamine glycerophospholipids and phosphatidylinositol. Furthermore, macrophages from caveolin-1 null mice show decreased AA mobilization and prostaglandin E(2) and LTB(4) production upon cell stimulation. Collectively, these results provide insight into the role of caveolin-1 in AA homeostasis and suggest an important role for this protein in the eicosanoid biosynthetic response.