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Cold Spring Harbor Laboratory Press, Genes & Development, 10(21), p. 1163-1168, 2007

DOI: 10.1101/gad.431507

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Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores

Journal article published in 2007 by Emma M. J. King, Najma Rachidi, Nick Morrice, Kevin G. Hardwick, Michael J. R. Stark ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The spindle checkpoint delays anaphase onset until all chromosomes are correctly attached to microtubules. Ipl1 protein kinase (Aurora B) is required to correct inappropriate kinetochore–microtubule attachments and for the response to lack of tension between sister kinetochores. Here we identify residues in the checkpoint protein Mad3p that are phosphorylated by Ipl1p. When phosphorylation of Mad3p at two sites is prevented, the cell’s response to reduced kinetochore tension is dramatically curtailed. Our data provide strong evidence for a distinct checkpoint pathway responding to lack of sister kinetochore tension, in which Ipl1p-dependent phosphorylation of Mad3p is a key step.