Parallel Incorporation of Different Fluorinated Amino Acids: On the Way to “Teflon” Proteins
Journal article published in 2010 by
Lars Merkel,
Melina Schauer,
Garabed Antranikian,
Nediljko Budisa 
This paper is made freely available by the publisher.
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Abstract
(Chemical Equation Presented) Nonstick proteins? We report the successful residue-specific fluorination of 24 positions in a lipase by simultaneous incorporation of three different noncanonical amino acids. The enzyme produced in a polyauxotrophic E. coli strain structurally tolerates all fluorine atoms and retains its catalytic activity very well.