Published in

Elsevier, Methods in Cell Biology, p. 301-315, 2015

DOI: 10.1016/bs.mcb.2015.03.003

Links

Tools

Export citation

Search in Google Scholar

Time-lapse recording of centrosomes and other organelles in Drosophila neuroblasts

Journal article published in 2015 by Judit Pampalona, Jens Januschke, Paula Sampaio, Cayetano Gonzalez ORCID
Distributing this paper is prohibited by the publisher
Distributing this paper is prohibited by the publisher

Full text: Unavailable

Red circle
Preprint: archiving forbidden
Red circle
Postprint: archiving forbidden
Red circle
Published version: archiving forbidden
Data provided by SHERPA/RoMEO

Abstract

Drosophila larval neuroblasts (NBs) are an excellent model for asymmetric division and cell cycle studies in general. For decades, visualizing relevant structures like centrosomes, chromosomes, or the mitotic spindle relied exclusively on immunofluorescence on fix samples. More recently, improvements on sensitivity and acquisition speed of different confocal systems have made it possible to acquire time-resolved images of combined fluorescent reporters from single larval NBs. Here, we provide protocols to visualize centrosomes and other organelles from both primary cultures of isolated single NBs and ex vivo, whole-mounted larval brains.