Objective. To assay anti-ganglioside antibodies (aGM 1 ) in sera of a large cohort of European patients with systemic lupus erythematosus (SLE) to define the prevalence of these autoantibodies in SLE; to evaluate the association of aGM 1 with clinical manifestations and other autoantibodies found in SLE; and to search for aGM 1 association with HLA class II alleles. Methods. Four hundred forty-eight patients with SLE were consecutively enrolled in 8 centers from 6 European countries. All sera were tested for antinuclear antibodies by immunofluorescence on HEp-2 cells as substrate, anti-dsDNA, aGM 1 aCL, aβ 2 -glycoprotein I (aβ 2 -GPI) antibodies by ELISA, and antineutrophil cytoplasmic antibodies (ANCA) by immunofluorescence and by ELISA. Genomic typing for HLA class II loci was performed by polymerase chain reaction-sequence specific oligonucleotide probe method. Clinical assessment was done at the time of enrolment. Results. We found 41.9% of patients with clinical signs of neuropsychiatric involvement; 15.5% of patients were positive for aGM:1, 8% of the IgG isotype and 8.6% of the IgM isotype; aGM 1 - IgG were associated with neuropsychiatric manifestations (NPM) (RR = 3.7), with migraine (RR = 2.4), with OBS (RR = 7.3), and with peripheral neuropathy (RR = 8.5). aGM 1 -IgM were associated with NPM (RR = 4) and with depression (RR = 3.4). Furthermore, the genetic study showed that aGM 1 -IgG were associated with HLA-DQB1*0404 (RR = 7.2) while aGM 1 -IgM were associated with HLA-DQB1*0605 (RR = 33.3). No associations were found between aGM 1 and anti-dsDNA, aCL, aβ 2 GPI, or ANCA. Conclusion. Our results show aGM 1 can be found in patients with SLE. aGm 1 may play a pathogenetic role for some NPM in this condition.