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Elsevier, Forensic Science International: Genetics Supplement Series, 1(1), p. 19-21

DOI: 10.1016/j.fsigss.2007.10.111

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Single primer extension (SPEX) amplification to accurately genotype highly damaged DNA templates

Journal article published in 2008 by Paul Brotherton, Phillip Endicott, Mark Beaumont, Ross Barnett ORCID, Jeremy Austin, Alan Cooper, Juan J. Sanchez
This paper is available in a repository.
This paper is available in a repository.

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Abstract

High levels of non-authentic sequence data can be generated by traditional PCR-based methodologies when DNA is damaged, template numbers are small and/or the target amplification size too large. We therefore present an alternate methodology based on single primer extension (SPEX) amplification; that places no pre-defined size constraints on amplification and interacts with only one of the DNA strands at the target locus.