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Elsevier, Journal of Biotechnology, 2(99), p. 121-131

DOI: 10.1016/s0168-1656(02)00178-5

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Studies on the properties of Celluclast/Eudragit L-100 conjugate

Journal article published in 2002 by Fernando Dourado ORCID, Maria de Lurdes Bastos ORCID, M. Mota, F. M. Gama ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

A cellulase from Trichoderma reesei was immobilized on Eudragit L-100, a reversibly soluble polymer depending on the pH of the medium. The solubility of the modified cellulase was studied at different pH values. By changing the pH, the adsorption equilibrium of the derivatized proteins is switched towards the liquid phase, thus making recycling possible. This method allows for improved stability, without major loss of specific activity. The adsorption of cellulase on Eudragit lowers the enthalpy of denaturation, but affects only slightly the denaturation temperature. The use of carbodiimide was ineffective on linking the enzymes covalently to the polymer, since the immobilization process was found to be only mediated by non-covalent forces.