Published in
Elsevier, Journal of Microbiological Methods, (106), p. 101-103, 2014
DOI: 10.1016/j.mimet.2014.08.012
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An improved method for rapid generation and screening of Bacillus thuringiensis phage-resistant mutants.
Journal article published in 2014 by
Annika Gillis 
,
Jacques Mahillon
,
Jacques Mahillon
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Abstract
A simple method to isolate, screen and select phage-resistant mutants of Bacillus thuringiensis was developed. The traditional double-layer agar method was improved by a combination of the spotting assay using a lytic phage, to generate the bacterial-resistant mutants, with an inverted spotting assay (ISA), to rapidly screen the candidate-resistant mutants.