Microorganisms live in environments that are geometrically, physically and chemically heterogeneous at the micro-scale. Consequently, they have to optimise their behaviour at the cellular level within limited time frames by responding to stimuli that are spatially distributed at the micro-scale. Microorganisms achieve this by using a range of different sensing mechanisms as a means to gain biologically-critical and dynamic information about their physical (e.g., available space) and chemical (e.g., distribution of nutrients) environment. Despite this micro-scale dependent behaviour, the response of microorganisms is usually studied in and on homogeneous media, which have little resemblance to their natural environment. Semiconductor fabrication offers large possibilities for the design and creation of physically- and chemically-microstructured environments, which either mimic the natural ones, or represent a multitude of possible environments to be used in combinatorial experiments. In order to test the dynamic behaviour of microorganisms we propose here the design and fabrication of test microstructured arrays and demonstrate their applications using representative fungal (Neurospora crassa) and bacterial (Escherichia coli) species.