Neste trabalho estamos reportando o isolamento de cinco compostos de Aspergillus sp EJC08 isolado como endof?tico de Bauhinia guianensis uma planta t?pica da Amaz?nia. Os compostos ergosterol (1), per?xido de ergosterol (2), mevalolactona (3), monometilsulocrina (4) e tripacidina A (5) foram isolados atrav?s de procedimentos cromatogr?ficos e identificados por m?todos espectrais de RMN 1D e 2D e EM. Os compostos 3, 4 e 5 foram testados contra Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis e Staphylococcus aureus e mostraram boa atividade. ; ABSTRACT: The present work reports the isolation of five compounds from Aspergillus sp EJC08 isolated as endophytic from Bauhinia guianensis, a tipical plant of the Amazon. The compounds ergosterol (1), ergosterol peroxide (2), mevalolactone (3), monomethylsulochrin (4) and trypacidin A (5) were isolated by chromatographic procedures and identified by spectral methods of 1D and 2D NMR and MS. Compounds 3, 4 and 5 were tested against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus and showed good activity.