ABSTRACT We present a systematic study of mid-infrared spectra of galaxies including star-forming galaxies and active galactic nuclei observed with JWST MIRI-MRS and NIRSpec-IFU. We focus on the relative variations of the 3.3, 6.2, 7.7, 11.3, 12.7, and 17 $μ$m polycyclic aromatic hydrocarbon (PAH) features within spatially resolved regions of galaxies including NGC 3256, NGC 7469, VV 114, II Zw96, and NGC 5728. Using theoretical PAH models and extending our earlier work, we introduce a new PAH diagnostic involving the 17 $μ$m PAH feature. To determine the drivers of PAH band variations in galaxies, we compare observed PAH spectral bands to predictions from theoretical PAH models. We consider extinction, dehydrogenation, and PAH size and charge as possible drivers of PAH band variations. We find a surprising uniformity in PAH size distribution among the spatially resolved regions of the galaxies studied here, with no evidence for preferential destruction of the smallest grains, contrary to earlier findings. Neither extinction nor dehydrogenation play a crucial role in setting the observed PAH bands. Instead, we find that PAH charge plays a significant role in PAH inter-band variations. We find a tight relation between PAH charge and the intensity of the radiation field as traced by the [Ne iii]$/$[Ne ii] maps. In agreement with recent JWST results, we find a predominance of neutral PAH molecules in the nuclei of active galaxies and their outflows. Ionized PAHs are the dominant population in star-forming galaxies. We discuss the implications of our findings for the use of PAHs as ISM tracers in high redshift galaxies.