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Royal Society of Chemistry, Chemical Science, 31(15), p. 12534-12542, 2024

DOI: 10.1039/d4sc02904f

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Advancing the enzymatic toolkit for 2′-fluoro arabino nucleic acid (FANA) manipulation: phosphorylation, ligation, replication, and templating RNA transcription

Journal article published in 2024 by Yingyu Liu ORCID, Jun Wang ORCID, Yashu Wu, Yajun Wang ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Scheme of the amplification of DNA–FANA chimera via single-fNTP substitutional PCR, the synthesis of RNA from DNA–FANA chimeric templates via IVT, followed by the activation of the fluorescent spinach aptamer upon the addition of DFHBI.