Royal Society of Chemistry, Chemical Science, 31(15), p. 12534-12542, 2024
DOI: 10.1039/d4sc02904f
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Scheme of the amplification of DNA–FANA chimera via single-fNTP substitutional PCR, the synthesis of RNA from DNA–FANA chimeric templates via IVT, followed by the activation of the fluorescent spinach aptamer upon the addition of DFHBI.