AbstractBackgroundCholesterol in cell membranes is crucial for cell signaling, adhesion, and migration. Membranes feature cholesterol‐rich caveolae with caveolin proteins, playing roles in epithelial‐mesenchymal transition and cancer progression. Despite elevated cholesterol levels in tumors, its precise function and the effects of its depletion in oral squamous cell carcinoma remain unclear. The aim of this study was to evaluate the influence of cholesterol depletion in oral squamous cell carcinoma cell line and epithelial‐mesenchymal transition process.MethodsCholesterol depletion was induced on SCC‐9 cells by methyl‐β‐cyclodextrin and cell viability, proliferation, apoptosis, and colony formation capacities were evaluated. Gene and protein expressions were evaluated by reverse transcription polymerase chain reaction (RT‐qPCR) and Western Blot, respectively, and cell sublocalization was assessed by immunofluorescence.ResultsCholesterol depletion resulted in alteration of oral squamous cell carcinoma cell morphology at different concentrations of methyl‐β‐cyclodextrin, as well as decreased cell proliferation and viability rates. Analysis of CAV1 transcript expression revealed increased gene expression in the treated SCC‐9 during the 24 h period, at different concentrations of methyl‐β‐cyclodextrin: 5 , 7.5, 10, and 15 mM, in relation to parental SCC‐9. CAV1 protein expression was increased, with subsequent dose‐dependent decrease. A statistically significant difference was observed in samples treated with 5 mM of methyl‐β‐cyclodextrin (p = 0.02, Kruskal–Wallis test). The immunofluorescence assay showed lower cytoplasmic and membrane labeling intensity in the treated samples for CAV1.ConclusionThese findings indicate the modulation of cholesterol as a possible mechanism underlying the regulation of these molecules and activation of epithelial‐mesenchymal transition in oral squamous cell carcinoma.